Journal: The Journal of Neuroscience
Article Title: Drosophila Tachykininergic Neurons Modulate the Activity of Two Groups of Receptor-Expressing Neurons to Regulate Aggressive Tone
doi: 10.1523/JNEUROSCI.1734-22.2023
Figure Lengend Snippet: DNA sequence of 13LexAop2-IVS-TkR86C/TkR86C:HA constructs (pJFRC-13LexAop2-IVS-TkR86C/TkR86C:HA)
Article Snippet: The plasmid used for embryo injection has the fragment below inserted between NotI and XbaI sites of the plasmid pJFRC19-13×LexAop2-IVS-myr::GFP (catalog #32141, Addgene), which is the backbone common to all pJFRC reporter vectors ( ). NotI and XbaI sites are shown at the 5′ and 3′ end of the sequence, respectively. The HA tag sequence is present only in the 13XLexAop2-IVS-TkR86C:HA construct. The 3 point mutations are indicated in bold letters. .
Techniques: Sequencing, Construct, Plasmid Preparation, Injection